Development of Real-Time and Conventional PCR Assays for Identifying Stubby Root Nematode Paratrichodorus allius
نویسندگان
چکیده
منابع مشابه
Development of Conventional and Real-Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies.
Scabies remains the most prevalent, endemic, and neglected ectoparasitic infestation globally and can cause institutional outbreaks. The sensitivity of routine microscopy for demonstration of Sarcoptes scabiei mites or eggs in skin scrapings is only about 50%. Except for three studies using conventional or two-tube nested PCR on a small number of cases, no systematic study has been performed to...
متن کاملDevelopment of conventional and real-time multiplex PCR assays for the detection of nosocomial pathogens
Nosocomial infections are major clinical threats to hospitalised patients and represent an important source of morbidity and mortality. It is necessary to develop rapid detection assays of nosocomial pathogens for better prognosis and initiation of antimicrobial therapy in patients. In this study, we present the development of molecular methods for the detection of six common nosocomial pathoge...
متن کاملDevelopment of conventional and real-time PCR assays for the rapid detection of group B streptococci.
BACKGROUND Group B streptococci (GBS), or Streptococcus agalactiae, are the leading bacterial cause of meningitis and bacterial sepsis in newborns. Currently available rapid methods to detect GBS from clinical specimens are unsuitable for replacement of culture methods, mainly because of their lack of sensitivity. METHODS We have developed a PCR-based assay for the rapid detection of GBS. The...
متن کاملReal-time multiplex PCR assays.
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
متن کاملdevelopment and comparison of conventional pcr and sybr green real time pcr for detection of aggregatibacter actinomycetemcomitans and tannerella forsythensis
background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Disease
سال: 2017
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-10-16-1431-re